anti igf2 goat polyclonal antibody  (R&D Systems)

Journal: Frontiers in Veterinary Science

Article Title: Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy

doi: 10.3389/fvets.2022.912721

Figure Lengend Snippet: List of antibodies and IgGs used for immunohistochemistry of equine endometrium and conceptus membranes.

Article Snippet: IGF2 , Goat polyclonal anti-IGF2 , F20 sc-7435 Santa Cruz (1: 50) , Biotinylated rabbit anti-goat , BA-5000, Vector Laboratories Inc. , Goat IgG (1: 100).

Techniques: Immunohistochemistry, Control, Plasmid Preparation

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Imbalanced Expression of IGF2 and PCSK4 Is Associated With Overproduction of Big IGF2 in SFT With NICTH: A Pilot Study.

doi: 10.1210/jc.2018-00593

Figure Lengend Snippet: Figure 2. Serum IGF2 isoform analysis. (A) WIB analysis of serum IGF2 in eight patients with SFT. Patients #1 to #5 are patients with SFT without hypoglycemia, and patients #10 to #12 are patients with SFT and hypoglycemia. Molecular size markers (in kilodaltons) are indicated by lines on the left. (B) Comparison of big IGF2 production levels between the non-NICTH group and the NICTH group. (C) Comparison of mature IGF2 production levels between the non-NICTH group and the NICTH group. (D) Comparison of the big IGF2/mature IGF2 production-level ratio between the non-NICTH group and the NICTH group. P values were obtained using the t test; P , 0.05 was accepted as significant in all panels.

Article Snippet: Sections were also stained with an anti-IGF2 goat polyclonal antibody (AF-292-NA; R&D Systems, Minneapolis, MN) and an anti-PCSK4 rabbit polyclonal antibody (NBP1-88010; Novus Biologicals, Littleton, CO).

Techniques: Comparison

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Imbalanced Expression of IGF2 and PCSK4 Is Associated With Overproduction of Big IGF2 in SFT With NICTH: A Pilot Study.

doi: 10.1210/jc.2018-00593

Figure Lengend Snippet: Figure 3. Comparative analysis of gene-expression levels in SFT using qRT-PCR. (A) IGF2 mRNA expression levels in the non-NICTH group and the NICTH group. (B) PCSK4 mRNA expression levels in the non-NICTH group and the NICTH group. (C) The IGF2/PCSK4 mRNA expression-level ratio in the non-NICTH group and the NICTH group. *P = 0.093, **P = 0.217, ***P = 0.006. P values were obtained using the t test. These values are not statistically significant but tentative as a result of the limited number of samples.

Article Snippet: Sections were also stained with an anti-IGF2 goat polyclonal antibody (AF-292-NA; R&D Systems, Minneapolis, MN) and an anti-PCSK4 rabbit polyclonal antibody (NBP1-88010; Novus Biologicals, Littleton, CO).

Techniques: Gene Expression, Quantitative RT-PCR, Expressing

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Imbalanced Expression of IGF2 and PCSK4 Is Associated With Overproduction of Big IGF2 in SFT With NICTH: A Pilot Study.

doi: 10.1210/jc.2018-00593

Figure Lengend Snippet: Figure 4. Comparative analysis of protein expression levels in SFT by immunohistochemistry analysis. (A) Representative images of immunohistochemistry for IGF2. (B) Representative images of immunohistochemistry for PCSK4. (C) Comparison of IGF2 expression levels between the non-NICTH group and the NICTH group. (D) Comparison of PCSK4 expression levels between the non-NICTH group and the NICTH group. (E) Comparison of the IGF2/PCSK4 expression-level ratio between the non-NICTH group and the NICTH group. P values were obtained using the t test; (C and E) P , 0.05 was accepted as significant and is indicated in bold.

Article Snippet: Sections were also stained with an anti-IGF2 goat polyclonal antibody (AF-292-NA; R&D Systems, Minneapolis, MN) and an anti-PCSK4 rabbit polyclonal antibody (NBP1-88010; Novus Biologicals, Littleton, CO).

Techniques: Expressing, Immunohistochemistry, Comparison

Journal: Histopathology

Article Title: Distinguishing adrenal cortical carcinomas and adenomas: a study of clinicopathological features and biomarkers

doi: 10.1111/his.12283

Figure Lengend Snippet: Expression of miR483-3p, IGF 2 and Smad4 in adrenocortical tumors

Article Snippet: Sections were incubated at 4°C overnight with anti-IGF2 goat polyclonal antibody (sc-1415; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) diluted at 1:100, anti-MIB-1 monoclonal antibody working liquid (ZM-0167, ZSGB-BIO, China), or anti-Smad4 rabbit monoclonal antibody (ab40759; Abcam, Cambridge, MA, USA) diluted at 1:100.

Techniques: Expressing

Journal: Histopathology

Article Title: Distinguishing adrenal cortical carcinomas and adenomas: a study of clinicopathological features and biomarkers

doi: 10.1111/his.12283

Figure Lengend Snippet: Representative staining for miR483-3p, IGF2 and Smad4 in adrenal cortical tumours. MiR483-3p was detected by in-situ hybridization (ISH), and IGF2 and Smad4 by immunochemistry. A, MiR483-3p in an adrenocortical carcinoma (ACC). The miR483-3p ISH signal is expressed mainly in the cytoplasm in all tumour cells of the ACC. Positive staining has a blue–violet colour. B, Perinuclear dot-like and homogeneous cytoplasmic staining for IGF2 in all or nearly all tumour cells in the same ACC correlating with the expression of miR483-3p (4+ staining). C, Smad4 in an ACC showing negative staining (score 0). Note positive labelling in the non-neoplastic fibrous tissue on the left. D, Negative miR483-3p ISH in an ACA. E, IGF2 in an ACA showing 1+ staining. Note intra-cytoplasmic specks only. F, An ACA displaying strong, diffuse cytoplasmic and occasional nuclear expression of Smad4.

Article Snippet: Sections were incubated at 4°C overnight with anti-IGF2 goat polyclonal antibody (sc-1415; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) diluted at 1:100, anti-MIB-1 monoclonal antibody working liquid (ZM-0167, ZSGB-BIO, China), or anti-Smad4 rabbit monoclonal antibody (ab40759; Abcam, Cambridge, MA, USA) diluted at 1:100.

Techniques: Staining, In Situ Hybridization, Expressing, Negative Staining

Journal: Histopathology

Article Title: Distinguishing adrenal cortical carcinomas and adenomas: a study of clinicopathological features and biomarkers

doi: 10.1111/his.12283

Figure Lengend Snippet: Correlation between miR483-3p, IGF 2 or Smad4 expression and cellular proliferative activity

Article Snippet: Sections were incubated at 4°C overnight with anti-IGF2 goat polyclonal antibody (sc-1415; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) diluted at 1:100, anti-MIB-1 monoclonal antibody working liquid (ZM-0167, ZSGB-BIO, China), or anti-Smad4 rabbit monoclonal antibody (ab40759; Abcam, Cambridge, MA, USA) diluted at 1:100.

Techniques: Expressing, Activity Assay

Journal: Histopathology

Article Title: Distinguishing adrenal cortical carcinomas and adenomas: a study of clinicopathological features and biomarkers

doi: 10.1111/his.12283

Figure Lengend Snippet: Clinical features and immunohistochemical results of the 15 borderline tumors

Article Snippet: Sections were incubated at 4°C overnight with anti-IGF2 goat polyclonal antibody (sc-1415; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) diluted at 1:100, anti-MIB-1 monoclonal antibody working liquid (ZM-0167, ZSGB-BIO, China), or anti-Smad4 rabbit monoclonal antibody (ab40759; Abcam, Cambridge, MA, USA) diluted at 1:100.

Techniques: Immunohistochemical staining

Journal: Nucleus

Article Title: A mRNA and Cognate MicroRNAs Localize in the Nucleolus

doi: 10.4161/19491034.2014.990864

Figure Lengend Snippet: Nucleolus-enriched RNAs 1

Article Snippet: This was performed as previously detailed using a polyclonal rabbit IgG for IGF2 ( Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:25 dilution followed by visualization using a Cy3-conjugated donkey anti-rabbit IgG.

Techniques: Binding Assay, Sequencing

Journal: Nucleus

Article Title: A mRNA and Cognate MicroRNAs Localize in the Nucleolus

doi: 10.4161/19491034.2014.990864

Figure Lengend Snippet: Fluorescence in situ hybridization. - : Cells incubated in buffer prior to hybridization; + : cells pre-incubated with RNase. A: IGF2 mRNA hybridization probes. B: 28 S rRNA probes. C: Scrambled sequence probes (see Materials and Methods). The upper panels in A-C are phase contrast images, the lower panels are the fluorescence microscopy images. Arrows indicate hybridization to IGF2 mRNA in nucleoli. (See also Figure S2 ).

Article Snippet: This was performed as previously detailed using a polyclonal rabbit IgG for IGF2 ( Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:25 dilution followed by visualization using a Cy3-conjugated donkey anti-rabbit IgG.

Techniques: Fluorescence, In Situ Hybridization, Incubation, Hybridization, Sequencing, Microscopy

Journal: Nucleus

Article Title: A mRNA and Cognate MicroRNAs Localize in the Nucleolus

doi: 10.4161/19491034.2014.990864

Figure Lengend Snippet: Predicted binding sites of miR-206 in the 3′-UTR of IGF2 mRNA.

Article Snippet: This was performed as previously detailed using a polyclonal rabbit IgG for IGF2 ( Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:25 dilution followed by visualization using a Cy3-conjugated donkey anti-rabbit IgG.

Techniques: Binding Assay

Journal: Nucleus

Article Title: A mRNA and Cognate MicroRNAs Localize in the Nucleolus

doi: 10.4161/19491034.2014.990864

Figure Lengend Snippet: Predicted binding sites of miR-351 in the 3′-UTR of IGF2 mRNA.

Article Snippet: This was performed as previously detailed using a polyclonal rabbit IgG for IGF2 ( Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:25 dilution followed by visualization using a Cy3-conjugated donkey anti-rabbit IgG.

Techniques: Binding Assay

Journal: Nucleus

Article Title: A mRNA and Cognate MicroRNAs Localize in the Nucleolus

doi: 10.4161/19491034.2014.990864

Figure Lengend Snippet: Predicted binding sites of miR-494 and miR-340 in the 3′-UTR of IGF2 mRNA.

Article Snippet: This was performed as previously detailed using a polyclonal rabbit IgG for IGF2 ( Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:25 dilution followed by visualization using a Cy3-conjugated donkey anti-rabbit IgG.

Techniques: Binding Assay

Journal: Nucleus

Article Title: A mRNA and Cognate MicroRNAs Localize in the Nucleolus

doi: 10.4161/19491034.2014.990864

Figure Lengend Snippet: Predicted binding sites of miR-664 in the 5′- and 3'-UTRs of IGF2 mRNA.

Article Snippet: This was performed as previously detailed using a polyclonal rabbit IgG for IGF2 ( Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:25 dilution followed by visualization using a Cy3-conjugated donkey anti-rabbit IgG.

Techniques: Binding Assay

Journal: Nucleus

Article Title: A mRNA and Cognate MicroRNAs Localize in the Nucleolus

doi: 10.4161/19491034.2014.990864

Figure Lengend Snippet: Summary of predicted miRNA Targets in IGF 2 mRNA 1

Article Snippet: This was performed as previously detailed using a polyclonal rabbit IgG for IGF2 ( Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:25 dilution followed by visualization using a Cy3-conjugated donkey anti-rabbit IgG.

Techniques: Sequencing

Journal: Nucleus

Article Title: A mRNA and Cognate MicroRNAs Localize in the Nucleolus

doi: 10.4161/19491034.2014.990864

Figure Lengend Snippet: Comparison of the predicted rat myoblast miR-206:IGF2 mRNA interaction and that of miR-206 and KLF4 mRNA in human cells.

Article Snippet: This was performed as previously detailed using a polyclonal rabbit IgG for IGF2 ( Santa Cruz Biotechnology, Santa Cruz, CA) at a 1:25 dilution followed by visualization using a Cy3-conjugated donkey anti-rabbit IgG.

Techniques: Comparison

Journal: Journal of Biological Chemistry

Article Title: Inhibition of Apoptosis by Amphiregulin via an Insulin-like Growth Factor-1 Receptor-dependent Pathway in Non-small Cell Lung Cancer Cell Lines

doi: 10.1074/jbc.m207584200

Figure Lengend Snippet: FIG. 5. Effect of H358-conditioned medium, AR, and IGF1 on IGF1-R tyrosine phosphorylation. Serum-deprived H322 cells were incubated: A, for 30 min in serum-free medium (), in serum-free medium with IGF1 50 ng/ml (IGF1), or for indicated times in serum-free H358 CM (CM); B, for 30 min in serum-free medium (), in H358 CM (CM), in H358 CM preincubated with anti-AR neutralizing antibody (CM Ac AR), or in serum-free medium with 50 ng/ml of IGF1 (IGF1) or AR (AR), prior to detergent lysis and immunoprecipitation of the IGF1-R subunit. C, serum-deprived H358 cells were incubated for 30 min in serum-free medium (), in H358 CM (CM), or in serum-free medium with 50 ng/ml of IGF1 (IGF1) or AR (AR), prior to detergent lysis and immunoprecipitation of the IGF1-R subunit. Tyrosine phos- phorylation was visualized by protein immunoblotting with monoclonal anti-phosphotyrosine IgG as described (upper lane). Equal loading of immunoprecipitated proteins was confirmed by reprobing immunoblots with polyclonal anti-IGF1-R subunit antibody (lower lane). IgG, im- munoglobulin control for immunoprecipitation. Data are representative of at least three separate experiments.

Article Snippet: Anti-human EGF or AR mouse monoclonal, anti-human TGF , betacellulin, or IGF2 goat polyclonal (R&D Systems Europe, Ltd., Abingdon, UK) or anti-human IGF1 goat polyclonal (Oncogene Research Products, Fontenay sous Bois, France) neutralizing antibodies were incubated overnight at 4 °C in H358 CM.

Techniques: Phospho-proteomics, Incubation, Lysis, Immunoprecipitation, Western Blot, Control

Journal: Journal of Biological Chemistry

Article Title: Inhibition of Apoptosis by Amphiregulin via an Insulin-like Growth Factor-1 Receptor-dependent Pathway in Non-small Cell Lung Cancer Cell Lines

doi: 10.1074/jbc.m207584200

Figure Lengend Snippet: FIG. 6. Effect of EGFR inhibitors on IGF1-R tyrosine phosphorylation. Serum-deprived H322 cells were incubated for 30 min in serum-free medium (), in H358 CM (CM), or in serum-free medium supplemented with AR 50 ng/ml (AR) or with IGF1 50 ng/ml (IGF1), in the presence or absence of EGFR inhibitors AG556 20 (M) or ZD1839 (500 nM). Detergent lysis of proteins, immunoprecipitation of the IGF1-R subunit, and tyrosine phosphorylation Western blotting (upper lane) were then performed. Equal loading of immunoprecipitated proteins was confirmed by reprobing immunoblots with polyclonal anti-IGF1-R subunit antibody (lower lane). IgG, immunoglobulin control for immunopre- cipitation. Data are representative of three independent experiments.

Article Snippet: Anti-human EGF or AR mouse monoclonal, anti-human TGF , betacellulin, or IGF2 goat polyclonal (R&D Systems Europe, Ltd., Abingdon, UK) or anti-human IGF1 goat polyclonal (Oncogene Research Products, Fontenay sous Bois, France) neutralizing antibodies were incubated overnight at 4 °C in H358 CM.

Techniques: Phospho-proteomics, Incubation, Lysis, Immunoprecipitation, Western Blot, Control